Fig. 6.

Repression of c-Jun, JunD, and Fra-2 by shRNA in v-Src-transformed CEF. CEF were coinfected with the temperature-sensitive mutant NY72-4 RSV and a group B virus expressing a shRNA for c-jun, junD, or fra-2 or the control virus RCASBP(B)-shRNA-ΔU6 (Control). (A to C) Repression was determined at the nonpermissive and permissive temperatures of 41.5°C and 37.5°C, respectively, by Western blotting followed by densitometry. Erk-1 was used as a loading control. (D and E) AP-1 activity was determined by transient expression assays under conditions of JunD, Fra-2, or c-Jun downregulation by shRNA.