Fig. 7.
An NS5A species encoding a Y93H substitution is refractory to relocalization. Recombinant lentiviruses were designed to express WT NS5A-GFP (i) or Y93H NS5A-GFP (ii) (AH, amphipathic helix) (A) and used to infect Replicon 1b cells (B and C, respectively). Following infection, cells were treated with 1% DMSO or 10× the IC50 of NS5Ai 1 for 48 h and examined for GFP fluorescence using live-cell confocal microscopy (i and ii, respectively, in B and C). Boxed regions in i and ii (B and C) are magnified in the bottom left corner of their respective images. Arrowheads indicate LDs.