Fig. 1.

NSs downregulates p62 in whole-cell lysates. (A) 293 cells were mock infected or infected with MP-12 at an MOI of 3 and harvested at the indicated time points postinfection. Whole-cell lysates were analyzed by Western blotting with anti-p62, anti-RVFV, and anti-β-actin antibodies (from top to bottom). The asterisk denotes a nonspecific band. (B) 293 cells were transfected with in vitro-synthesized RNA for GFP or NSs and harvested 8 h posttransfection. Whole-cell lysates were analyzed by Western blotting with anti-p62, anti-RVFV, anti-GFP, and anti-β-actin antibodies (from top to bottom). Representative examples of at least 3 independent experiments are shown.