Fig. 6.

NSs interacts with p62. (A) Schematic representation of the S segment of the recombinant virus (rMP-12-NSs-SF) used in the experiments whose results are shown in panel B. SF, Strep-Tag II–FLAG tag. (B) 293 cells were mock infected or infected with rMP12-NSs-SF at an MOI of 3. At 5 h.p.i., NSs-SF was affinity precipitated with Strep-Tactin magnetic beads. Whole-cell lysates and the precipitated proteins (STREP) were analyzed by Western blotting with anti-p62, anti-FLAG, and anti-β-actin antibodies (from top to bottom). Representative examples of at least 3 independent experiments are shown.