Fig. 2.

Percentages of neurons expressing GFP following infection with HSV1-VP26-GFP or HSV2-VP26-GFP. Neuronal cultures were infected with HSV1-VP26-GFP or HSV2-VP26-GFP (at an MOI of 100, 30, or 10) for 10, 15, or 24 h and were assayed for GFP expression as a marker of productive infection. Both viral constructs express a VP26-GFP fusion protein during active viral assembly, permitting GFP visualization during productive infection. Results reflect data from six separate experiments for each virus for the 10- and 15-h time points and from two experiments for each virus for the 24-h time point. For each time point, 2,636 to 12,027 neurons were assayed.