Fig. 3.

LGP2 overexpression in LGP2 TG mice does not modify basal gliosis and monocyte influx into the brain. (A) Astrogliosis was assayed by immunohistochemistry on noninfected WT and LGP2 TG paraffin-embedded brain sections, using a GFAP primary Ab. Numbers of GFAP⁺ cells in the caudal diencephalon were 44.6 ± 4.2 and 51.3 ± 3.1 for WT and LGP2 TG mice, respectively (20 fields were counted per mouse type). (B) Flow cytometry of cells isolated from the NS of WT and LGP2 TG mice by use of a Percoll gradient and double stained with CD11b and CD45 Abs to analyze monocyte infiltration and microglia activation. The CD11b⁺ cells correspond to infiltrated monocytes (9.7 and 7.5% in WT and LGP2 TG mice, respectively), and the CD11b⁺ CD45^{+ intermediate} cells are microglia (1% in both types of mice). Results are representative of two independent experiments.