Fig. 6.

Innate immune impairment does not modify immune parameters in the periphery. (A) RABV-specific neutralizing Ab titers (expressed in international units per ml [IU/ml]) in the blood were compared by the rapid fluorescent-focus inhibition technique, using WT and LGP2 TG mouse sera taken at 11 days p.i. (n = 9 and 8, respectively). (B) LGP2 TG and WT mice exhibited similar spleen subpopulations. Spleen cells of RABV-infected WT and LGP2 TG mice (n = 4) were taken at days 5 and 8 p.i. and stained with paired Abs for CD4 and CD8 or B220 and CD19. Percentages of CD4⁺ CD8⁺ and CD19⁺ B220⁺ cell populations were analyzed by flow cytometry. Results are presented as CD4⁺/CD8⁺ ratios and percentages of B220⁺ CD19⁺ cells (n = 8).