Fig. 3.
Identification of the WSSV IE1 domain that interacts with PmTBP. (A) Coimmunoprecipitation of PmTBP-FLAG with IE1-V5, EGFP-V5, and two EGFP-V5 fusion proteins, containing the IE1 N-terminal transactivation domain and the IE1 C-terminal DNA binding domain (EGFP-IE11–80-V5 and EGFP-IE181–224-V5, respectively). In the lower two panels, Western blot analysis confirms the expression of the inputs in cell lysates from cotransfected Sf9 cells. (B) Schematic of the modular organization of the WSSV IE1 protein and a summary of the interaction domain analysis results.