Fig. 5.
Identification of the PmTBP domain that interacts with WSSV IE1. (A) Coimmunoprecipitation of IE1-FLAG with EGFP-V5 and two EGFP-V5 fusion proteins, containing the PmTBP N-terminal variable region and the PmTBP C-terminal DNA binding domain (EGFP-PmTBP1–110-V5 and EGFP-PmTBP111–300-V5, respectively). In the lower panels, Western blot analysis confirms the expression of inputs in cell lysates from cotransfected Sf9 cells. (B) Schematic of the modular organization of the full-length PmTBP protein and a summary of the interaction domain analysis results.