Fig. 8.

dsRNA knockdown of WSSV ie1 and PmTBP expression. (A) Semiquantitative RT-PCR detection of WSSV ie1 expression in gills of L. vannamei shrimp at 24 and 48 h post-WSSV challenge [i.e., 72 and 96 h after pretreatment with the indicated dsRNA(s)]. Shrimp EF-1α was included as a template control. RT-PCR products were analyzed in a 1.5% agarose gel. (B) Real-time RT-PCR quantification of expression levels of PmTBP after dsRNA pretreatment and WSSV challenge. Transcripts were measured in duplicate for every individual shrimp (n = 4 [or 3] for each experimental condition) and normalized against EF-1α. Values are means ± SEM. Statistically significant differences are indicated by different letters (A and B) (P < 0.05).