Table 2.
Primers used to construct full-length and truncated IE1 and PmTBP expression plasmids
| Plasmid | Primer name (sequence [5′–3′]a) |
|---|---|
| pDHsp-IE1-V5-His | IE1-F (CCCAAGCTTCTCAAGATGGCCTTTAATTTTG) |
| IE1-R (TCCCCGCGGTACAAAGAATCCAGAAATCTCA) | |
| pDHsp-PmTBP-V5-His | PmTBP-F (CGCGGATCCATGGATCACATGCTGCCTTCCCCG) |
| PmTBP-R (TCCCCGCGGTTGTTTTTTGAAGCTTTTTAATAT) | |
| pDHsp-IE1-FLAG-His | IE1-F/IE1-R |
| pDHsp-PmTBP-FLAG-His | PmTBP-F/PmTBP-R |
| pDHsp-EGFP-V5-His | EGFP-F (CCCAAGCTTACCATGGTGAGCAAGGGCGAGGAG) |
| EGFP-R (CGCGGATCCCTTGTACAGCTCGTCCATGCC) | |
| pDHsp-EGFP-IE11–80-V5-His | IE1-1-F (CGCGGATCCATGGCCTTTAATTTTGAAGAC) |
| IE1-80-R (TCCCCGCGGGAGATTCTCCATATCTTCTGC) | |
| pDHsp-EGFP-IE181–224-V5-His | IE1-81-F (CGCGGATCCAACAGTGGTTCCCATGTCAAG)/IE1-R |
| pDHsp-EGFP-IE181–120-V5-His | IE1-81-F/IE1-120-R (TCCCCGCGGGTCCATGTATTGGGGAAAAGT) |
| pDHsp-EGFP-IE1101–140-V5-His | IE1-101-F (CGCGGATCCAACTGGATTGAAGAGACTGAT) |
| IE1-140-R (TCCCCGCGGCCTAGCCATCCATTTCATGGC) | |
| pDHsp-EGFP-IE1121–160-V5-His | IE1-121-F (CGCGGATCCGGTGGGGATGGTTCACGTGGG) |
| IE1-160-R (TCCCCGCGGTATGGATGCCGCATTTTCTAC) | |
| pDHsp-EGFP-IE1141–180-V5-His | IE1-141-F (CGCGGATCCGATGTGACTTTCTTTGTGTTTG) |
| IE1-180-R (TCCCCGCGGATTGTCAACAATCACCTTTAC) | |
| pDHsp-EGFP-IE1161–200-V5-His | IE1-161-F (CGCGGATCCTGGATGTACCAAAAACTACTA) |
| IE1-200-R (TCCCCGCGGCACTGGGCCTGGGTACTTGCA) | |
| pDHsp-EGFP-IE1181–224-V5-His | IE1-181-F (CGCGGATCCGCATCAAACCCAATGTTTTCT)/IE1-R |
| pDHsp-EGFP-PmTBP1–110-V5-His | PmTBP-F/PmTBP-110-R (TCCCCGCGGGGGCCCTCCTATGGGGGTGGAA) |
| pDHsp-EGFP-PmTBP111–300-V5-His | PmTBP-F (CGCGGATCCATGACCCCCATGACCCCACAC)/PmTBP-R |
| pDHsp-EGFP-PmTBP111–170-V5-His | PmTBP-111-F/PmTBP-170-R (TCCCCGCGGCCGTGGTTCTCGAATACGCAT) |
| pDHsp-EGFP-PmTBP141–200-V5-His | PmTBP-141-F (TCCCCGCGGGAGCTGAAGAAAATTGCACTC) |
| PmTBP-200-R (TCCCCGCGGCGCATACTTTCTTGCCGCAAG) | |
| pDHsp-EGFP-PmTBP171–230-V5-His | PmTBP-171-F (TCCCCGCGGACAACAGCCCTCATCTTCTCC) |
| PmTBP-230-R (TCCCCGCGGGATAGGGAACTTAACGTCACA) | |
| pDHsp-EGFP-PmTBP201–260-V5-His | PmTBP-201-F (TCCCCGCGGAGAATAGTGCAAAAGCTTGG) |
| PmTBP-260-R (TCCCCGCGGAGGCTTGACCATTCGGTAGAT) | |
| pDHsp-EGFP-PmTBP231–300-V5-His | PmTBP-231-F (TCCCCGCGGCGATTGGAGGGTCTTGTTCTC)/PmTBP-R |
| pET-28b-PmTBP | PmTBP-BamHI-F (CGCGGATCCAAGCATGGATCACATGCTGCCT) |
| PmTBP-XhoI-R (CCGCTCGAGTTTTTTGAAGCTTTTTAATATA) | |
| pAcMNPVie1Pro-V5-His | AcMNPV ie1-F (GGAATTCCATATGTCGATGTCTTTGTGATGCGCG) |
| AcMNPV ie1-R (CCCAAGCTTAGTCACTTGGTTGTTCACGATC) | |
| pAcMNPVie1Pro-PmTBP-V5-His | PmTBP-F/PmTBP-R |
a
Restriction enzyme cutting sites are underlined.