Table 3.

Primer sequences used for dsRNA production, RT-PCR, and real-time RT-PCR analysis

Gene name	Primer name (sequence [5′–3′])	Usage
ie1	IE1-F (GAAGACTCTACAAATCTCTTT)	dsRNA production
	IE1-R (CTTGCACCTACACGCATTACA)	dsRNA production
	IE1-T7-F (GGATCCTAATACGACTCACTATAGGGAAGACTCTACAAATCTCTTT)	dsRNA production
	IE1-T7-R (GGATCCTAATACGACTCACTATAGGCTTGCACCTACACGCATTACA)	dsRNA production
PmTBP	PmTBP-F (CTGGAATGTTCGGTTCAGCC)	dsRNA production
	PmTBP-R (GGCTTCGTAAATCTGGTTTC)	dsRNA production
	PmTBP-T7-F (GGATCCTAATACGACTCACTATAGGCTGGAATGTTCGGTTCAGCC)	dsRNA production
	PmTBP-T7-R (GGATCCTAATACGACTCACTATAGGGGCTTCGTAAATCTGGTTTC)	dsRNA production
EGFP	EGFP-F (ATGGTGAGCAAGGGCGAGG)	dsRNA production
	EGFP-R (TTACTTGTACAGCTCGTCCAT)	dsRNA production
	EGFP-T7-F (GGATCCTAATACGACTCACTATAGGATGGTGAGCAAGGGCGAGG)	dsRNA production
	EGFP-T7-R (GGATCCTAATACGACTCACTATAGGTTACTTGTACAGCTCGTCCAT)	dsRNA production
ie1	CGCGGATCCATGGCCTTTAATTTTGAAGAC	RT-PCR
	TCCCCGCGGTACAAAGAATCCAGAAATCTC	RT-PCR
EF-1α	GGAGATGCACCACGAAGCTC	RT-PCR
	TTGGGTCCGGCTTCCAGTTC	RT-PCR
PmTBP	GCAGCAGGGTCAGCAGATG	Real-time RT-PCR
	CATTAAGGTGTGTGGCGTGAAC	Real-time RT-PCR
dnapol	TCCAGCTCCATCACCCTCTT	Real-time RT-PCR
	TGAATTTGGAATTGGTGTCGTAGA	Real-time RT-PCR
EF-1α	TGCTCTGGACAACATCGAGC	Real-time RT-PCR
	CGGGCACTGTTCCAATACCT	Real-time RT-PCR