Fig. 2.

RV infection or poly(I:C) treatment stimulates ROS generation in polarized airway epithelial cells. Polarized 16HBE14o− cells were infected with RV or UV-RV (A and B) or treated with poly(I:C) (C and D). Generation of ROS was measured by flow cytometry using carboxy-H₂DCFDA 24 h after RV infection (A) or 8 h after poly(I:C) treatment (C). The kinetics of ROS generation was measured in RV-infected (B) or poly(I:C)-treated (D) cells by flow cytometry. Histograms are representative of four independent experiments. Data in panels B and D are means ± SEM for four independent experiments carried out in duplicate. *, different from medium control or UV-RV-treated cells (P ≤ 0.05 by ANOVA).