Fig. 5.

CXCR3 is required for ASC accumulation in the CNS. (A) ELISPOT assay-derived frequencies of virus-specific and total IgG-ASC in spinal cords of WT and CXCR3^−/− mice at days 21 and 28 p.i. (B) Representative flow cytometry density plots of CD138⁺ ASC derived from spinal cords of WT and CXCR3^−/− mice at day 21 p.i. (left) and a representative bar graph depicting reduced frequencies of CD138⁺ ASC in spinal cords of CXCR3^−/− mice (right). Data are representative of three experiments with 4 to 6 mice/time point. (C) Virus-specific IgM and IgG2a in supernatants from spinal cord homogenates of infected WT and CXCR3^−/− mice assessed by ELISA (left and middle panels). Arbitrary units reflect total Ab levels per spinal cord from three individual mice per time point. Background values from naïve mice were subtracted. IgG mRNA values represent average expression levels relative to GAPDH ± standard error of the mean from three individual mice/time point (right panel).