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. 2011 Jul;85(13):6275–6286. doi: 10.1128/JVI.02125-10

Fig. 2.

Fig. 2.

Polymerase activity of reassortant viral polymerase complexes. The replication and transcription activity of reconstituted viral polymerase complexes in which the PB2, PB1, and/or PA genes of CA04 (H1N1) were replaced with cognate polymerase subunits from human, avian, and swine influenza viruses. (A) Single-gene reassortants; (B) double-gene reassortants; (C) triple-gene reassortants. Luciferase-based minigenome reporter assays were used to measure polymerase activity in the background of a WT CA04 (H1N1) NP plasmid. Activity values shown are the means from at least three assays. The first row at the bottom lists the recombinants by number (“r” denotes reverse genetics). Shaded reassortants were efficiently rescued. Asterisks indicate P values (*, P < 0.05; **, P < 0.005) that represent a statistically significant difference from the polymerase activity of the reconstituted viral polymerase of the homogeneous WT CA04 (H1N1) complex (100%), the baseline for comparison (as determined by two-tailed paired t tests). Error bars indicate standard deviations of the means. Virus abbreviations: A/California/04/09, CA04; A/Puerto Rico/8/34, PR8; A/Sw/Korea/CAN01/04, CAN01; A/Cheongju/H407/08, H407; A/Sw/Korea/JNS06/04, JNS06; A/Dk/Korea/LPM91/06, L91; A/Em/Korea/W150/06, W150; A/Ab/Korea/W81/05, W81; A/Ab/Korea/ma81/07, ma81; A/Ab/Korea/ma81K/07, ma81K; A/Ab/Korea/W44/05, W44; A/Ab/Korea/ma44/07, ma44; A/Md/Korea/6L/07, 6L; A/Ck/Korea/04116/04, 04116; A/Ck/Korea/04164/04, 04164.