Table 1.
Viruses used in this studya
| Virus strain | Subtype | Host | Abbreviation | PB2 alteration at: |
PB1-F2 protein expression | PA alteration at aa 97 | |
|---|---|---|---|---|---|---|---|
| aa 627 | aa 701 | ||||||
| A/California/04/09 | H1N1 | Human | CA04 (H1N1) | E | D | No | T |
| A/Puerto Rico/8/34 | H1N1 | Human | PR8 (H1N1) | K | D | Yes | T |
| A/Sw/Korea/CAN01/04 | H1N1 | Swine | Sw/CAN01 (H1N1) | E | D | Yes | T |
| A/Cheongju/H407/08 | H3N2 | Human | H407 (H3N2) | K | D | Yes | T |
| A/Sw/Korea/JNS06/04 | H3N2 | Swine | Sw/JNS06 (H3N2) | E | D | Yes | T |
| A/Dk/Korea/LPM91/06 | H3N2 | Avian | Av/LPM91 (H3N2) | E | D | Yes | T |
| A/EM/Korea/W150/06 | H5N1 | Avian | Av/W150 (H5N1)b | K | D | Yes | T |
| A/Ab/Korea/W81/05 | H5N2 | Avian | Av/W81 (H5N2) | E | D | Yes | T |
| A/Ab/Korea/ma81/07 | H5N2 | ma | Av/ma81 (H5N2) | E | D | Yes | I |
| A/Ab/Korea/ma81K/07 | H5N2 | ma | Av/ma81K (H5N2)c | K | D | Yes | I |
| A/Ab/Korea/W44/05 | H7N3 | Avian | Av/W44 (H7N3) | E | D | Yes | T |
| A/Ab/Korea/ma44/07 | H7N3 | ma | Av/ma44 (H7N3) | K | D | Yes | I |
| A/Md/Korea/6L/07 | H7N6 | Avian | Av/6L (H7N6) | E | D | Yes | T |
| A/Ck/Korea/04116/04 | H9N2 | Avian | Av/04116 (H9N2) | E | D | Yes | T |
| A/Ck/Korea/04164/04 | H9N8 | Avian | Av/04164 (H9N8) | E | D | Yes | T |
These human, swine, and avian influenza viruses were used to generate polymerase reassortant viruses with the pandemic (H1N1) 2009 virus (CA04). Accession numbers for the viral polymerase genes (PB2, PB1, and PA) used are the following: Sw/CAN01 (H1N1), EU798918, EU798898, and EU798878; H407 (H3N2), FJ009502, FJ009455, and FJ009455; Sw/JNS06 (H3N2), EU301177, EU301400, and EU301368; Av/LPM91 (H3N2), EU301207, EU301430, and EU301398; Av/W150 (H5N1), EU233746, EU233745, and EU233744; Av/W81 (H5N2), GU361390, GU361308, and GU361350; Av/ma81 (H5N2), HQ913050 (note that A/Ab/Korea/ma81K/07 differs from A/Ab/Korea/ma81/07 only by the presence of the E627K mutation in PB2; therefore, PB1 and PA for these two viruses are the same); Av/W44 (H7N3), HQ913044, HQ913051, and HQ913057; Av/ma44 (H7N3), HQ913048, HQ913055, and HQ913061; Av/6L (H7N6), HQ913047, HQ913054, and HQ913060; Av/04116 (H9N2), HQ913045, HQ913052, and HQ913058; Av/04164 (H9N8), HQ913046, HQ913053, and HQ913059. Abbreviations: aa, amino acid; ma, mouse adapted; Ab, aquatic bird; Ck, chicken; Dk, duck; EM, environment; Md, mallard; Sw, swine.
Highly pathogenic H5 avian influenza virus (29).
This virus contains PB2627K as an additional adaptive mutation.