Table 1.
Previously characterized viral virion proteins
| Protein content of PRV virions | Common name | Proposed function(s) | Virion localization | Mass (kDa) | Detection by nLC-ESI/MALDIc | Presence in HSV-1d | Reference(s) |
|---|---|---|---|---|---|---|---|
| EP0a | ICP0 | Gene regulation (transactivator) | Unknown | 44 | +/− | + | 17, 108 |
| IE180 | ICP4 | Gene regulation (transactivator) | Tegument | 150 | +/+ | + | 99 |
| pORF1.2 | Unknown | Unknown | 35 | +/− | NAb | 113 | |
| pUL1a | gL | Viral entry (fusion) | Envelope | 17 | +/+ | + | 68 |
| pUL3.5 | Secondary envelopment and egress | Tegument | 24 | +/+ | − | 38 | |
| pUL6 | Portal protein | Capsid | 70 | +/+ | + | 114 | |
| pUL7 | Interacts with mitochondrial ANT2 | Tegument | 29 | +/− | + | 37 | |
| pUL10 | gM | Secondary envelopment and egress | Envelope | 42 | +/+ | + | 32 |
| pUL11a | Secondary envelopment and egress | Tegument | 7 | +/− | + | 75 | |
| pUL16 | Interacts with UL21 | Tegument | 35 | +/+ | + | 93 | |
| pUL17 | DNA cleavage and encapsidation | Capsid | 64 | +/+ | + | 71 | |
| pUL18 | VP23 | Minor capsid protein | Capsid | 32 | +/+ | + | 114 |
| pUL19 | VP5 | Major capsid protein | Capsid | 146 | +/+ | + | 114 |
| pUL21 | Interacts with UL16 | Tegument | 55 | +/+ | + | 29, 98 | |
| pUL22 | gH | Viral entry (fusion) | Envelope | 72 | +/+ | + | 74 |
| pUL25 | Capsid assembly | Capsid | 57 | +/+ | + | 59 | |
| pUL26 | VP24 | Minor capsid scaffold (protease) | Capsid | 55 | +/+ | + | 114 |
| pUL27 | gB | Viral entry (fusion) | Envelope | 100 | +/+ | + | 28 |
| pUL35 | VP26 | Surface capsid protein | Capsid | 11 | +/+ | + | 123 |
| pUL36 | VP1/2 | Viral entry and egress | Tegument | 324 | +/+ | + | 24 |
| pUL37 | Viral egress | Tegument | 98 | +/+ | + | 73 | |
| pUL38 | Minor capsid protein | Capsid | 40 | +/+ | + | 114 | |
| pUL39 | RR1 | Ribonucleotide reductase | Tegument | 91 | +/+ | − | 122 |
| pUL41 | VHS | Degrades host and viral mRNAs | Tegument | 41 | +/+ | + | 82 |
| pUL43 | Inhibits membrane fusion | Envelope | 38 | +/− | − | 66 | |
| pUL44 | gC | Viral entry (virion attachment) | Envelope | 51 | +/+ | + | 13 |
| pUL46 | VP11/12 | Unknown | Tegument | 76 | +/+ | + | 76 |
| pUL47 | VP13/14 | Secondary envelopment and egress | Tegument | 80 | +/+ | + | 76 |
| pUL48 | VP16, α-TIF | Gene regulation (transactivator) | Tegument | 45 | +/+ | + | 38 |
| pUL49 | VP22 | Major tegument protein | Tegument | 25 | +/+ | + | 39 |
| pUL49.5 | gN | Immune evasion | Envelope | 10 | +/+ | − | 57 |
| pUL51 | Secondary envelopment and egress | Tegument | 25 | +/+ | + | 81 | |
| pUL53 | gK | Secondary envelopment and egress | Envelope | 34 | +/− | − | 67 |
| pUS2 | Tegument protein | Tegument | 28 | +/+ | + | 23 | |
| pUS3 | PK | Protein kinase, inhibits apoptosis | Tegument | 43 | +/+ | + | 47 |
| pUS4 | gG | Immune evasion, secreted | Envelope | 53 | +/− | + | 116 |
| pUS6 | gD | Viral entry | Envelope | 45 | +/+ | + | 13 |
| pUS7 | gI | Cell-cell spread | Envelope | 39 | +/+ | + | 13 |
| pUS8 | gE | Cell-cell spread | Envelope | 62 | +/+ | + | 13 |
| pUS9 | Axonal sorting | Envelope | 11 | +/− | + | 15 | |
| Putative false negatives | |||||||
| pUL13 | VP18.8 | Protein kinase | Tegument | 41 | −/− | + | 27, 106 |
| pUL56 | ORF1 | Unknown | Envelope | 22 | −/− | + | 62 |
a
Detected in virions not treated with proteinase K.
b
No known HSV-1 orthologue.
c
+/+, detected by both nLC-ESI and MALDI-based proteomic workflows; +/−, identified by only nLC-ESI workflow; −/−, absent in both nLC-ESI and MALDI workflows.
d
As determined by MS (83).