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Effect of neutralizing antibody on infection spread in RNA-transfected cells. Cells were monitored in the presence (A) and in the absence (B) of neutralizing anti-CVB3 MAb. At 24 h and 48 h p.i., the cells were fixed and immunolabeled with anti-CVB3 Ab, followed by Alexa 488-labeled anti-rabbit IgG (green). DIC and inverted gray scale images are shown. Scale bars, 50 μm.
