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. 2011 Jul;85(13):6784–6794. doi: 10.1128/JVI.00485-11

Fig. 2.

Fig. 2.

Interactions between HCpro and the translation initiation factors of tobacco and potato using the GAL4 YTHS. Two variants (a and b) of the translation initiation factors eIF(iso)4E and eIF4E (4E) from tobacco (Tiso4Ea and Tiso4Eb; T3Ea and T4Eb) and potato (Piso4Ea and Piso4Eb; P3Ea and P4Eb) were tested for interactions with the HCpro of PVA. HCpro and initiation factors were fused to the AD or BD and were expressed following cotransformation of yeast cells with the respective vector plasmids. (A) Growth of yeast (Saccharomyces cerevisiae AH109) 10 days after plating on stringent selective medium lacking adenine, histidine, leucine, and tryptophan indicates an interaction between the tested proteins. T4Ea and T4Eb autoactivated the reporter genes when fused to the BD vector and were not included in the assay. Western blot analysis with monoclonal antibodies specific to the AD (B) or the BD (C) was used to detect the expressed proteins. Coomassie blue-stained SDS-PAGE gel served as a protein loading control.