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. 2011 Jul;85(13):6784–6794. doi: 10.1128/JVI.00485-11

Fig. 3.

Fig. 3.

Detection of protein-protein interactions in leaves of N. benthamiana using BiFC. HCpro proteins of three potyviruses, PVA, PVY, and TEV, were expressed with YN fused to the HCpro N terminus. Translation initiation factors eIF(iso)4E (Tiso4Eb and Piso4Eb) and eIF4E (T4Ea and P4Eb) of tobacco (T) and potato (P) were expressed with YC fused to the N or C terminus. Leaves were infiltrated with pairs of Agrobacterium strains expressing tester proteins tagged with the opposite halves of YFP (the corresponding bacterial cultures were mixed in a 1:1 ratio for infiltration). YFP fluorescence indicating protein-protein interactions was detected in epidermal cells using an epifluorescence microscope 3 days postinfiltration. Images were taken using the same exposure time. For detection of the expressed recombinant proteins using protein-specific antibodies, see Fig. S1 in the supplemental material.