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. 2011 Jul;85(13):6784–6794. doi: 10.1128/JVI.00485-11

Fig. 4.

Fig. 4.

Detection of YFP fluorescence resulting from interaction between PVA HCpro and Tiso4Eb, and its association with 6K2-induced viral vesicles in N. benthamiana. (A) YN-HCpro was expressed from an engineered infectious clone of PVA during the course of virus infection, and Tiso4Eb-YC was coexpressed from a binary vector under 35S promoter. The fluorescence resulting from the interaction between HCpro and Tiso4Eb fluorescence was partly confined to round structures or bodies. Scale bars represent 10 μm. (B) Schematic presentations of the constructs used for protein coexpression. The mature viral proteins: P1, the first protein; HC-Pro; P3, third protein; 6K1 and 6K2, 6-kDa proteins; CI, cylindrical inclusion protein; VPg; NIaPro, the main viral proteinase; NIb, replicase; and CP, coat protein. AA indicates the poly(A) tail. The 5′ and 3′ untranslated regions are not depicted. (Ci to iii) Localization of interacting HCpro and Tiso4E with 6K2-induced viral vesicles. Arrows highlight fluorescence signals and colocalization. Confocal images are single optical sections selected from z-series for viewing with LAS AF Lite.