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. 2011 Jul;85(13):6784–6794. doi: 10.1128/JVI.00485-11

Fig. 5.

Fig. 5.

The putative 4E binding protein (4E-BP) motif of HCpro. (A) The amino acids 345 and 351 of PVA HCpro correspond to the conserved residues in the 4E-BP consensus motif YXXXXLΦ, where X is a variable amino acid and Φ is a hydrophobic residue, and were substituted for alanine (underlined) in the PVA HCpro [HC4Emut (MUT)]. (B) Interactions of the wild-type (WT) HCpro of PVA and HC4Emut (MUT) with the eIF(iso)4E of tobacco (Nicotiana tabacum) (Tiso4Eb) illustrated by the β-galactosidase enzyme activity in cotransformed yeast (96-well microtiter plate format) following overnight incubation. The results from three technical replicates of four independent yeast colonies are shown. (+) and (-), the positive and negative control provided by Clontech, respectively. (C) Mean relative β-galactosidase activities corresponding to the samples shown in panel B and quantified by Image J (National Institutes of Health). Standard deviation is indicted with error bars.