Table 1.
Systemic infections of plants of Nicotiana tabacum and N. benthamiana with PVA-HC4Emut that carries two amino acid substitutions (Y345A and L350A) in the putative 4E-binding site of HCpro, or with the nonmutated Potato virus A (wt PVA)a
| Strain | Expt 1 |
Expt 2 |
Expt 3 |
||||||
|---|---|---|---|---|---|---|---|---|---|
| 7 dpi | 11 dpi | 21 dpi | 7 dpi | 11 dpi | 21 dpi | 7 dpi | 11 dpi | 21 dpi | |
| N. tabacum | |||||||||
| PVA-HC4Emut | 0/5 | 0/5 | 0/5 | 0/5 | 0/5 | 1b/5 | 0/4 | 0/4 | 0/4 |
| wt PVA | 5/5 | 5/5 | 5/5 | 5/5 | 5/5 | 5/5 | 4/4 | 4/4 | 4/4 |
| N. benthamiana | |||||||||
| PVA-HC4Emut | 0/3 | 0/3 | 0/3 | 0/4 | 0/4 | 0/4 | 0/4 | 0/4 | 0/4 |
| wt PVA | 3/3 | 3/3 | 3/3 | 4/4 | 4/4 | 4/4 | 4/4 | 4/4 | 4/4 |
a
The number of systemically infected plants out of the number of inoculated plants. The upper noninoculated leaves were tested for PVA by DAS-ELISA at 7, 11, and 21 days dpi.
b
The HCpro-encoding sequence in the progeny viruses was amplified by immunocapture-RT-PCR, and the introduced mutations were verified by sequencing.