Fig. 2.

Autophagy induced by PR8 infection proceeds without a block in the pathway. (A) B6-IEd fibroblasts stably expressing GFP-LC3 (left) or GFP alone (right) were infected with PR8 (4 to 50 HAU/10⁶ cells), and GFP fluorescence was assessed via flow cytometry. (B) GFP-LC3-expressing fibroblasts were infected with PR8 in the presence or absence of the chemical inhibitor wortmannin, and GFP fluorescence was assessed. (C) B6-IEd fibroblasts were infected with PR8 as described for panel B, and autophagy was assessed by probing lysates for LC3 conversion by Western blotting. Results are representative of three independent experiments.