Table 2.
Enhancement of dauer arrest by heat shock promoter::ins-1 transgenes
| Dauer constitutive mutation
|
Transgenea
|
Phenotype at 20°C (%)
|
Phenotype at 25°C (%) after heat shock
|
Phenotype at 25°C (%)
|
||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Dauer
|
L1 arrest
|
N
|
Dauer
|
L1 arrest
|
N
|
Dauer
|
L1 arrest
|
N
|
||
| Wild type | epIs6 | n.d. | n.d. | n.d. | 1.6 | 0 | 248 | n.d | n.d. | n.d. |
| Wild type | epIs7 | n.d | n.d. | n.d. | 0 | 1.9 | 264 | n.d. | n.d. | n.d. |
| Wild type | none | n.d. | n.d. | n.d. | 0 | 0 | 291 | n.d. | n.d. | n.d. |
| daf-2(e1370) | epIs6 | 1.9 | 0 | 209 | 4.5 | 95.5 | 156 | 13.3 | 86.7 | 196 |
| daf-2(e1370) | epIs7 | 13.3 | 0 | 195 | 5.5 | 94.5 | 238 | 6.7 | 93.3 | 194 |
| daf-2(e1370) | none | 0 | 0 | 256 | 84.3 | 15.7 | 229 | 88.8 | 11.2 | 143 |
| daf-2(m41) | epIs6 | 13.1 | 0 | 268 | 100 | 0 | 127 | n.d. | n.d. | n.d. |
| daf-2(m41) | epIs7 | 100 | 0 | 402 | 100 | 0 | 107 | n.d. | n.d. | n.d. |
| daf-2(m41) | none | 1.9 | 0 | 155 | 100 | 0 | 87 | n.d. | n.d. | n.d. |
| daf-7(e1372) | epIs6 | 0 | 0.7 | 293 | 100 | 0 | 90 | n.d. | n.d. | n.d. |
| daf-7(e1372) | epIs7 | 78.6 | 0 | 248 | 100 | 0 | 71 | n.d. | n.d. | n.d. |
| daf-7(e1372) | none | 0 | 0 | 117 | 100 | 0 | 67 | n.d. | n.d. | n.d. |
(n.d.) Not determined.
a
epIs6 and epIs7 are independent integrated heat shock promoter::ins-1 transgenes.