Table 3.
Deletion of ins-1 does not affect dauer arrest
| Genotype
|
Phenotype at 20°C (%)
|
Phenotype at 26°C (%)
|
||||||
|---|---|---|---|---|---|---|---|---|
| L4 larvae and adult
|
Dauer
|
Other
|
Na
|
L4 larvae and adult
|
Dauer
|
Other
|
Na
|
|
| Wild type | 100 | 0 | 0 | 1501 | 99.2 | 0 | 0.8 | 1468 |
| ins-1(nr2091) | 99.8 | 0 | 0.2 | 1237 | 100 | 0 | 0 | 1762 |
| daf-2(e1370) | 86.8 | 7.5 | 5.7 | 967 | 0 | 100 | 0 | 792 |
| daf-2(e1370); ins-1(nr2091) | 91.9 | 6.3 | 1.8 | 2127 | 0 | 100 | 0 | 1109 |
| daf-2(e1365) | 99.1 | 0 | 0.9 | 1169 | 0.1 | 99.9 | 0 | 1091 |
| daf-2(e1365); ins-1(nr2091) | 99.0 | 0 | 1.0 | 1215 | 0 | 100 | 0 | 1218 |
| daf-7(e1372) | 42.3 | 52.2 | 5.5 | 1856b | 0 | 99.8 | 0.2 | 578 |
| daf-7(e1372; ins-1(nr2091)) | 37.0 | 58.3 | 4.7 | 1545b | 0 | 93.8 | 6.2 | 533 |
a
Total number of animals scored. Numbers represent the summary of at least two separate experiments with each genotype, except as noted.
b
Numbers represent the summary of at least two separate trials from three independent outcrossed lines of this genotype. During the course of this project we found a high degree of variability in the percentage of dauers formed at 20°C by daf-7 strains from different sources. Combining data from three independent isolates of each genotype demonstrated that variability among the lines was not a result of the ins-1 mutation.