Table 5.
Modulation of dauer arrest induced by ins-1 and human insulin by daf-2 and daf-7 pathway mutants
| Dauer defective mutation
|
Transgene
|
Phenotype at 26°C (%)a
|
|||
|---|---|---|---|---|---|
| L3 and L4 larvae and adult
|
Dauer
|
Otherb
|
Nc
|
||
| Wild type | control | 95.4 | 0 | 4.6 | 521 |
| daf-16(mgDf47) | control | 95.0 | 0 | 5.0 | 380 |
| daf-3(mgDf90) | control | 90.6 | 0 | 9.4 | 416 |
| daf-16(mgDf47); daf-3(mgDf90) | control | 79.8 | 16.5 | 3.6 | 689 |
| Wild type | ins-1d | 84.1 | 12.5 | 3.4 | 615 |
| daf-16(mgDf47) | ins-1 | 82.0 | 7.7 | 10.3 | 534 |
| daf-3(mgDf90) | ins-1 | 89.3 | 6.5 | 4.2 | 742 |
| daf-16(mgDf47); daf-3(mgDf90) | ins-1 | 84.6 | 9.1 | 6.3 | 760 |
| Wild type | human insulind | 54.3 | 35.4 | 10.3 | 477 |
| daf-16(mgDf47) | human insulin | 83.6 | 10.0 | 6.4 | 659 |
| daf-3(mgDf90) | human insulin | 85.8 | 2.7 | 11.5 | 550 |
| daf-16(mgDf47); daf-3(mgDf90) | human insulin | 80.3 | 2.9 | 16.8 | 720 |
To maximize the synchronicity of the progeny, parents were allowed to lay eggs for 2 h at 25°C.
This category includes animals that could not be classified as either dauers or L3 and older at the time of scoring, due to less synchronous growth of the progeny than in the other sets of experiments.
Total number of transgenic animals scored after 50 h at 26°C. Numbers represent the summary of at least two separate trials from a single transgenic line of each genotype.
In this set of experiments, we found that both transgenes caused a somewhat higher level of dauer formation in the wild-type background than the experiments shown in Table 2, perhaps due to fluctuations in incubator temperature between the two sets.