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. 2011 Jul;85(13):6319–6333. doi: 10.1128/JVI.02627-10

Fig. 6.

Fig. 6.

Autophagy machinery is required for HBV envelopment. (A) Huh7 cells were transfected and treated as described for Fig. 5A. The abundance of extracellular or intracellular enveloped virions and intracellular nucleocapsids was analyzed by EPA as described in Materials and Methods. dsDNA, double-stranded DNA; IP, immunoprecipitation. (B) The percentage of the inhibitory effect of 3-MA (% of control) on the abundance of extracellular or intracellular enveloped virions and intracellular nucleocapsids was calculated as described for Fig. 5B. Results represent the mean data from two independent experiments. (C) Huh7 cells were transfected as described for Fig. 5G. The abundance of extracellular or intracellular enveloped virions and intracellular nucleocapsids was analyzed by EPA as described in Materials and Methods. siEGFP, siBeclin1, and siATG5 indicate siRNA complexes targeting EGFP, Beclin1, and ATG5, respectively. (D) The percentage of the inhibitory effect of siRNA duplexes targeting Beclin1 or ATG5 (% of control) on the abundance of extracellular or intracellular enveloped virions and intracellular nucleocapsids was calculated as described for Fig. 5B. Results represent the mean data from two independent experiments. (E) pHBV1.3-ENV− was transfected into Huh7 cells. The cell lysates were precipitated with antibodies against HBsAg or HBcAg, respectively, and then subjected to EPA as described in Materials and Methods.