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RNA structures in FMDV and SVDV 3′NCRs enhance IFN-β induction. SK-6 cells were transfected with 20 μg/ml of FMDV and SVDV 3′NCR transcripts (denatured or renatured as described in Materials and Methods), with 10 μg/ml of poly(I:C) or 10 μg of tRNA. The fold induction of IFN-β in RNA-transfected cells above that in mock-transfected cells was determined by RT-qPCR normalized to GAPDH. Error bars show standard deviations between triplicates.
