Table 2.
RNA | Activity at time point (h postinoculation): |
|||
---|---|---|---|---|
4 | 8 | 24 | 48 | |
S | 2 | 128 | 162 | <2 |
IRES | 64 | 128 | 8 | <2 |
3′NCR | <2 | 32 | <2 | ND |
Poly(I:C) | 8 | 8 | <2 | <2 |
Groups of four to five mice were inoculated with 100 μg of FMDV transcripts or poly(I:C). Sera were collected and pooled at the indicated times postinoculation for each RNA. Antiviral activity is expressed as the reciprocal of the highest dilution of sera able to suppress VSV-induced cytopathic effect on L-929 cells in 50% of the wells assayed. No antiviral activity was detected in the pools of sera collected at 0 h postinoculation (<2). ND, not done.