Fig. 8.

GagZip G2A forms cytoplasmic capsid-like structures and can be found colocalized with cytoplasmic ABCE1. (A) Infected COS-1 cells expressing GagZip G2A were analyzed by TEM. (Left and center panels) Regions of plasma membranes (indicated by black arrowheads) from two adjacent cells. Capsid-like structures were not found at the plasma membrane but were found in the cytoplasm (indicated by white arrowheads). (Right panel) Additional regions of the cytoplasm containing capsid-like structures. Bars, 200 nm. (B) Infected COS-1 cells expressing GagZip G2A were also subjected to immunogold labeling, as for Fig. 6A to C, with Gag labeled by 6-nm-diameter gold particles and ABCE1 labeled by 15-nm-diameter gold particles. Panel 1 shows a representative region of the plasma membrane. Panels 2 to 5 show areas of the cytoplasm. White arrowheads indicate clusters of Gag colocalized with ABCE1 in the cytoplasm. Black arrowheads point to the plasma membrane. Boxes indicate large clusters of Gag colocalized with ABCE1, with underlying areas of electron density that are ≥100 nm in diameter. Bars, 200 nm.