Fig. 2.

The conditioned medium of Zta-expressing NPC cells enhances IL-10 production from monocytes. (A) NPC-TW01 cells and HONE-1 cells were transfected with a vector plasmid (TW01-Vec and HONE-Vec) or a Zta-expressing plasmid (TW01-Zta and HONE-Zta). An ELISA was used to measure IL-10 concentrations in their conditioned medium (CM alone) or in the final supernatants of THP-1 cells that were cultured with the indicated NPC cell-conditioned medium (THP-1 + CM). (B) Quantitative RT-PCR was used to measure the relative levels of IL-10 mRNA in THP-1 cells that were cultured with the indicated NPC cell-conditioned medium. (C) An ELISA was used to measure IL-10 concentrations in the final supernatants of U937 cells that were cultured with the indicated NPC cell-conditioned medium (U937 + CM).