Fig. 4.

CypA stimulation of domain II RNA binding requires isomerase activity. (a) GST-CypA binding assays were performed as described in the legend to Fig. 1. Mutant forms of NS5A domain II were generated by QuikChange mutagenesis (primer sequences are available upon request). (b) RNA binding assays were performed as described in the legend to Fig. 2. Where indicated (+), GST-CypA (wild type [WT] or H126Q) were included at 100 nM.