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. 2001 Mar 15;15(6):710–723. doi: 10.1101/gad.194101

Figure 6.

Figure 6

MBD2 is required for full repression of methylated templates. The amount of luciferase activity recovered from a methylated plasmid is expressed as a percentage of that recovered from an unmethylated plasmid (Mean ±s.e.). The names and genotypes of the cell lines used are indicated below the histograms. (a) MBD2-deficient cell lines fail to fully repress the SV40 early promoter or the murine phosphoglycerate kinase (Pgk) promoter when fully methylated. (b) Repression of the methylated SV40 early promoter is restored in the Mbd2(−/−) cell lines by cotransfection of plasmids producing either full-length MBD2 or MBD2(153–414). Repression is not restored by expression of MBD2(163–414) protein, full-length MBD3 or short-form MBD3 proteins, none of which selectively bind methylated DNA in vivo. Proteins encoded by the expression constructs are diagrammed to the right of the histogram.