Fig. 8.

Latency versus lytic replication viral gene expression levels in lymphoid and nonlymphoid tissues during WD-MCF. PBMC and mononuclear cells were isolated from pLN, spleen, liver, lung, and kidney of rabbits mock infected or infected with the 247Nluc⁺-excised strain and developing WD-MCF. (A) Multicolor flow cytometry staining of IgM⁺, T lymphocyte⁺, and CD8⁺ cells. The data of one rabbit representative of two independent experiments are shown (n = 3 animals). (B) Relative RNA expression of ORF73, ORF50, ORF25, and LUC. Expression levels normalized to 10⁶ copies of the HPRT housekeeping gene are shown. Bars show individual measurements from three rabbits and are representative of results from two independent experiments. (C) RNA expression of ORF50 expressed as ratios over ORF73 expression. RNA was extracted from BT cells 96 h p.i. with 10⁻³ PFU/cell. Bars show means ± SEM (n = 3 animals). Statistical analyses by two-tailed unpaired t test: **, P < 0.01.