Table 1.

Oligonucleotides used for PCRs

Sequence name	Primer	Primer sequence	Amplicon length (bp)	Reference
247N galK	247N-L-galKF	5′-AAGAAGTTGCAAGTCTCTCATCTGATAGCATAGTAAAAATCAGTTCAGTACCTGTTGACAATTAATCATCGGCA-3′	1,331
	247N-R-galKR	5′-TAAGTACTGATGGCTTGTCTTGTGTCACGATAGTTTAGAAAAAATCTGTATCAGCACTGTCCTGCTCCTT-3′	1,331
247N luc	247N-L-lucF	5′-AAGAAGTTGCAAGTCTCTCATCTGATAGCATAGTAAAAATCAGTTCAGTACGGTACCGAGATCTGCGATCTGC-3′	3,546
	247N-R-lucR	5′-TAAGTACTGATGGCTTGTCTTGTGTCACGATAGTTTAGAAAAAATCTGTACGATTTTACCACATTTGTAGAGG-3′	3,546
ORF3	ORF3F	5′-GGGCTAATTTGTGCAGTTTGTGA-3′	111	40
	ORF3R	5′-AGGTGTTTCTGAAAAGAGGGGAA-3′	111	40
Beta-globin	GLOBF	5′-GGTATCCTTTTTACAGCACAAC-3′	178	43
	GLOBR	5′-CAGGTCCCCAAAGGACTCG-3′	178	43
247N	pre-247Nluc	5′-CATTTTAAAATAGCTTGTCGGG-3′	568
	post-247Nluc	5′-GACATGGTCTTCTGTTAAAGTC-3′	568
ORF73	ORF73F	5′-GGACTAGACCCTCTTTATGACCC-3′	244	12
	ORF73R	5′-GCAATGGGTTCCTATTTTGCTCG-3′	244	12
ORF50	C500-3	5′-TCTGGCCCGTGCTGCAGCAAGACTCTCAG-3′	274	23
	C500-4	5′-TATAGTAGAATCCCGTCTGAGTGGTAGCTG-3′	274	23
ORF25	ORF25F	5′-GGCCGATGTGGCCTATTTTC-3′	465
	ORF25R	5′-TCGTCCAAGAGTACACGGTG-3′	465
luc	luc-int F	5′-GCCATTCTATCCGCTGGAAG-3′	641
	luc-int R	5′-TAGGATCTCTGGCATGCGAG-3′	641
HPRT	HPRT-F	5′-TGATAGATCCATTCCTATGACTGTAGA-3′	265	19
	HPRT-R	5′-GGGTCCTTTTCACCAGCAG-3′	265	19