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. 2011 Jul;85(14):7411–7418. doi: 10.1128/JVI.00265-11

Fig. 1.

Fig. 1.

Adaptation of SAFV-2 to high-titer growth in mammalian cells. (A) Mock-infected LLCMK2 rhesus monkey kidney cells showing normal morphology. (B) SAFV-2-infected LLCMK2 cells at 8 days postinfection (LLCMK2P8) showing small clusters of rounded cells slightly above the plane of the monolayer. (C) Temporal analysis of SAFV RNA replication in LLCMK2 cells at passage 6 by real-time RT-PCR showing a 2-log-unit increase in viral copy numbers. PI, postinfection. (D) Scheme of SAFV-2 adaptation in each cell line with the number of passages and development and progression of CPE over time. P1→P8, passage 1 to passage 8; P3, passage 3; cpe, cytopathic effect; d7, day 7. (E) Mock-infected U118 MG cells showing normal cell morphology. (F) SAFV-2-infected LLCMK2 P8 at 3 day p.i. showing advanced CPE at passage 7. (G) Mock-infected HeLa cells showing normal cell morphology. (H) SAFV-2 (U118 P13)-infected HeLa cells (24 h p.i.) showing advanced CPE at passage 3.