Fig. 4.

SAFV-2 plaque morphology, cell association, and sialic acid binding. (A and B) Plaques produced in BHK-21 cells by SAFV-2 strain GDVII after 3 days and BeAn after 4 days of incubation, respectively. (C) SAFV-2 plaques in HeLa cells after 4 days of incubation. Note that the SAFV-2 lysate was not plaque purified and shows mainly large and some small plaques. (D) Cell association determined from the ratio of the total virus yield in PFU (cells and supernatant) to that in the supernatant for GDVII, BeAn, and Saffold virus 2 (SafV-2). Values are means plus standard deviations (error bars) (SD). The graph shows the results of a representative experiment of 4 experiments. (E) Binding of [S³⁵]methionine-labeled virions to cells in suspension after treatment with 1 mU/ml of Clostridium perfringens neuraminidase (Neur.) for 45 min at 37°C or with buffer alone. Values are mean ± SD. The graph shows the results of a representative experiment of 3 experiments.