Fig. 4.

Posttranslational modifications affecting the binding of PG9 and PG16. (a) Western blot of gp140 Env immunoprecipitated by PG9 or PG16 from transfection supernatants containing Q259d2.17 or Q259d2.17 N160K gp140. Input, supernatant was directly loaded into the indicated wells: Beads, supernatants were incubated with protein G agarose beads in the absence of MAb. (b) Western blot of either uncleaved (gp140F) or cleaved (gp140C) gp140 Env proteins immunoprecipitated from transfection supernatants containing either SF162 or SF162 K160N. Molecular masses are indicated on the vertical axis.