Table 1.
PG9 Fab binding to gp140 by SPRa
| gp140 | gp140 monomer |
gp140 trimer |
||||
|---|---|---|---|---|---|---|
| KD (nM) | ka (M−1 s−1) | kd (s−1) | KD (nM) | ka (M−1 s−1) | kd (s−1) | |
| Q168a2 | 26.81 (3) | 1.731 (2) × 104 | 4.640 (2) × 10−4 | 20.21 (3) | 1.950 (2) × 104 | 3.940 (2) × 10−4 |
| Q259d2.17 | 29.30 (3) | 9.658 (9) × 103 | 2.830 (2) × 10−4 | Signal < 10 RU | Signal < 10 RU | Signal < 10 RU |
| Q461e2 | ND | ND | ND | 130.3 (2) | 1.557 (2) × 104 | 2.029 (2) × 10−3 |
| Q769h5 | Signal < 10 RU | Signal < 10 RU | Signal < 10 RU | Signal < 10 RU | Signal < 10 RU | Signal < 10 RU |
| SF162 K160N | 33.24 (4) | 1.746 (2) × 104 | 5.805 (4) × 10−4 | 26.46 (4) | 1.855 (2) × 104 | 4.910 (4) × 10−4 |
| SF162 | ND | ND | ND | Did not bind | Did not bind | Did not bind |
a
The standard error on the last significant figure is shown in parentheses. ND, not determined.