Figure 6.
Ectopic Brat suppresses growth of the wing. (A) The distribution of Brat in third instar imaginal discs of the indicated genotypes. In addition to endogenous protein, the discs in the center bear ectopic Brat in the posterior compartment (to the right). Analysis by confocal microscopy reveals that Brat is cytoplasmic (not shown). The hemizygous bratk06028 disc (right) shows a very low level of residual staining, demonstrating specificity of the antibody. (B) Western blot of wing imaginal disc extracts prepared from flies of the genotypes indicated above. (Lanes 3–5) Three different amounts of wild-type extract (1×, 2×, 4×); (lanes 4,–7) Extracts from two different UAS transgenic lines. (C) Adult wings from flies of the indicated genotype. Also shown is a tracing of the en>brat+ wing showing the average intervein area for this genotype as a percentage of wild type. (D) High magnification views of wild-type and en>brat+ wings. Each cell secretes a small hair that is visible in the micrographs; note that the cell density is the same.