Fig. 7.
DA and GDVII virus infection and pDAL and pGDVIIL transfection in BHK-21 cells induce similar amounts of cleaved caspase-3 but do not induce TUNEL-positive staining. (A) Western blots of infected lysates harvested 9 h following infection were immunostained with anti-cleaved caspase-3 antibody; the lysates were also probed for VP1 and β-actin. (B) Western blots of lysates transfected with constructs expressing DA or GDVII L were immunostained with anti-cleaved caspase-3 antibody; the same lysates were also probed for β-actin. Cells infected with wt DA or GDVII virus (C) or transfected with pDAL or pGDVIIL (D) were TUNEL stained. There was no significant increase in TUNEL staining in TMEV-infected or TMEV L-transfected cells above the level with mock infection (P > 0.05).