Fig. 2.

Identification of MAbs that bind heterologous HCV genotypes using yeast display of E2 protein. The E2 ectodomain gene from six strains corresponding to HCV genotypes 1 to 6 was cloned into the PYD1 vector and expressed on the surface of yeast (see Materials and Methods). Yeasts expressing HCV E2 were incubated with MAb supernatants, and binding was assessed by flow cytometry. Representative histograms from all neutralizing MAbs (H77.16, H77.28, H77.31, H77.39, H77.56, J6.27, J6.36, and J6.103; solid black histograms) and negative-control MAb (WNV E16; unfilled gray histograms) are depicted. The data are representative of at least three independent experiments.