Table 2.

Summary of MAb binding to genotype 1 mutants expressed on the surface of yeast

MAb	Bindinga
	G406D	G406S	N410Y	I411N	N415Y	T416I	N417T	G470A	W529R	G530A	G530D	D533N	R543G	C552S	G406S + G530A	G406D + G530D
H77.14	100	100	100	100	67	100	58	<1	100	91	100	100	66	100	100	100
H77.16	<1	58	<1	<1	76	97	100	100	100	97	100	100	100	100	<1	<1
H77.23	100	100	100	100	75	100	61	<1	100	100	100	100	89	100	100	100
H77.27	77	91	100	97	64	100	80	81	<1	<1	11	27	84	100	<1	<1
H77.28	96	100	100	74	38	96	57	74	100	61	100	76	<1	>500	100	100
H77.31	77	100	100	100	96	100	65	100	<1	<1	24	<1	100	100	<1	<1
H77.36	97	88	100	92	100	100	98	100	1.2	26	<1	80	100	100	21	<1
H77.39	100	100	100	100	<1	81	<1	61	100	100	100	100	50	100	100	100
H77.56	100	100	100	100	100	100	100	100	100	100	100	92	100	9.5	100	100

^aThe values shown were obtained by dividing the total fluorescence product (percent positive population × mean fluorescence intensity) of a mutant for a given MAb by the total fluorescence product of the wild-type E2 for a given MAb. This value was then divided by the total fluorescence product of a mutant for an oligoclonal MAb pool and by the total fluorescence product of WT E2 for the same oligoclonal pool (to control for E2 binding) and multiplied by 100. The values in boldface indicate complete loss of binding, with reductions in MAb binding greater than or equal to 80% for a given mutation. The underlined values indicate a partial reduction in binding, between 50 and 79%. The italicized, boldface, and underlined value shows enhancement of binding greater than 500%. The results are the averages of three independent experiments for each mutant and each antibody.