Table 3.

Summary of MAb binding to genotype 2a mutants expressed on yeast

MAb	Bindinga
	G397E	F403L	G406C	S440P	Y443C	A524V	W529C	E531V	R572S	H617L	G397E + R572S
J6.1	71	97	100	100	83	83	100	88	100	100	61
J6.2	71	100	16	100	93	73	20	68	42	6	56
J6.6	64	100	26	41	100	6	4	69	100	100	100
J6.15	100	100	100	75	85	66	13	34	100	100	79
J6.16	100	100	100	76	100	80	100	86	100	100	100
J6.27	74	67	100	90	95	<1	<1	77	83	100	80
J6.30	85	100	100	100	75	58	88	82	100	3	100
J6.36	100	<1	<1	60	95	80	100	100	100	100	<1
J6.39	94	91	100	100	83	100	3	63	100	100	81
J6.40	98	100	32	32	100	75	63	65	40	15	80
J6.60	51	58	100	<1	<1	65	90	81	70	73	77
J6.75	100	100	100	100	100	100	100	100	100	100	100
J6.85	100	100	100	100	91	72	10	27	100	100	80
J6.101	83	100	43	100	100	100	57	91	82	3	50
J6.103	100	2	<1	26	100	100	100	100	100	100	<1

^aThe values shown were obtained by dividing the total fluorescence product (percent positive population × mean fluorescence intensity) of a mutant for a given MAb by the total fluorescence product of the wild-type E2 for a given MAb. This value was then divided by the total fluorescence product of a mutant for an oligoclonal pool of MAbs and by the total fluorescence product of WT E2 for the oligoclonal pool (to control for E2 binding) and multiplied by 100. The values in boldface indicate complete loss of binding, with reductions in MAb binding greater than or equal to 80% for a given mutation. The underlined values show partial loss of binding, with a reduction between 50 and 79%. The results are the averages of three independent experiments for each mutant and each antibody. Polyprotein amino acid numbering was determined by alignment with the H77 strain using the Sequence Location tool on the Los Alamos HCV database (http://hcv.lanl.gov/cgi-bin/LOCATE/locate.cgi).