Table 1.
Pseudoreversions selected with replication-defective NS4B mutantsa
| Primary mutation | Pseudoreversion | Nucleotide exchangeb |
|---|---|---|
| F211A | L208F | CTT→TTT |
| T221N | ACT→AAT | |
| ±NS5A K139E | AAA→GAAc | |
| R214A | R192G | CGC→GGC |
| P220A | G200E | GGC→GAA |
| I253M | ATA→ATG | |
| E226A | G200S | GGC→AGC |
| A201E | GCG→GAG | |
| +NS5A K139E | AAA→GAAd |
a
Primary NS4B mutations and corresponding pseudoreversions are given.
b
That is, the nucleotide substitution detected in the cloned cDNA.
c
Mutation detected in a fraction of analyzed DNA clones.
d
Mutation observed in all analyzed DNA clones.