Fig. 1.
Preferential infection of cells that have internalized high levels of virus following exposure to cell-associated HIV-1. (A) Donor cells are discriminated from target cells using CellTrace Far Red-stained Jurkat HIV(Red)/Gag-iGFP donor cells and CellTracker Blue-stained primary CD4+ T cells (targets). (B) Target cells are flow sorted after 3 h of coculture into GFP+ and GFP− populations. (C) GFP+ target cells expressing HIV(Red) 27 h after sorting. (D) GFP− target cells expressing HIV(Red) 27 h after sorting.