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. 2011 Jul;85(14):7203–7215. doi: 10.1128/JVI.00262-11

Fig. 1.

Fig. 1.

Position of the Y68A mutation and expression of pUL34 by cell lines. (A) Schematic diagrams of pUL34 showing the locations of relevant sequence features. Protein sequence of pUL34 is indicated as a bar with the N terminus at the left. Sequences in pUL34 that mediate nuclear envelope targeting of the NEC are indicated as a stippled region. Position of Y68 is indicated below the bar. Positions of conserved regions are indicated immediately below each of the bars. The conservation plot shows conservation of biochemical properties of amino acids using all available herpesvirus sequences aligned by using the program MUSCLE (13). (B) Expression of wild-type and Y68A mutant pUL34 by stable cell lines. Digital images of Western blots are shown. Vero cells (lanes 1 and 2) or cells stably expressing WT pUL34 (lane 3) or Y68A pUL34 (lane 4) were infected with WT HSV-1(F) (lane 1) or UL34-null vRR1072(TK+) virus (lanes 2 to 4). Blotted infected cell proteins were probed for either scaffolding protein (top) or pUL34 (bottom).