Fig. 7.

Wild-type pUL34 expression is required for normal localization of gE but not gD. Vero cells (A, B, E, and F), WT UL34-expressing cells (C), and Y68A UL34-expressing cells (D) were infected with HSV-1(F) (A and E) or UL34-null virus (B to D and F) for 16 h and then fixed and immunofluorescently stained for gE (A to D) or gD (E and F). Arrowheads in panel A point to junctional surfaces, where gE concentrates in wild-type infection. Arrowheads in panel B point to cytoplasmic membranes, where gE concentrates during UL34-null infection. Arrowhead in panel D points to the nuclear envelope.