Table 1.
Primers used for construction and analysis of mutant US9 BAC
| Primer name | Primer sequencea |
|---|---|
| US9R58StGm For | 5′-TCCTCGTACGCATGGGCCGCCAACAGTCGGTATTAAGGCGctagCGCAGACGCACCCGCTGCGTGCTCGGATCCTAGGGATAACAGGG-3′ |
| US9R58StGm Rev | 5′-GACAGGCGATCACCATGCCGACGCAGCGGGTGCGTCTGCGctagCGCCTTAATACCGACTGTTGTCTAGAGGCCGCGGCGTTG-3′ |
| Gm mid For | 5′-GGTCGTGAGTTCGGAGACGTAGC-3′ |
| Gm mid Rev | 5′-CACTACGCGGCTGCTCAAACC-3′ |
| R58St unique For | 5′-TCCTCGTACGCATGGGCCG-3′ |
| R58St unique Rev | 5′–GACAGGCGATCACCATGCCGAC-3′ |
| US9 test Fwd | 5′-AGTCACTGCGACCGCAACTTCC-3′ |
| US9 test Rev | 5′-AAGGCTGGGTGCAAATTGCGG-3′ |
a
Underlined sequences have homology to the US9 gene. Lowercase indicates nucleotides altered to create the R58Stop mutation and an NheI restriction enzyme cleavage site. Sequences not underlined have homology to the Sce-Gmr cassette.